FFmpeg losslessly converts all PNGs in a directory into a single lossless AVI video file by:

ffmpeg -framerate 5 -pattern_type glob -i '*.png' -c:v ffv1 out.avi

-framerate 5

show 5 PNG image frames per second.

-pattern_type glob -i '*.png'

collect all .png files in this directory

-c:v ffv1

use the lossless FFV1 codec

If you have problems playing back the .avi file, try omitting the -c:v ffv1 parameter. Don’t go below a framerate of about 3 frames/second because some viewers won’t work (e.g. VLC).

FFmpeg file globbing does NOT work on Windows, even with FFMPEG 4.x. The error on Windows is like:

Pattern type ‘glob’ was selected but globbing is not supported by this libavformat build

Windows FFmpeg users, consider using Windows Subsystem for Linux for file globbing with FFmpeg.