Basler Aptina 0134 flat field

These measurements were each taken with a C-mount size deep red filter inserted, held by hand on a LED diffuse source. Three measurements were taken for each camera, rotating the filter and camera to help eliminate flatness issues with the diffuser. Due to noise in the images inherent to the 100 ms exposure, a smoothing filter was used.

Gaussian smoothing kernel

Gaussian smoothing kernel

Deep-red filter

Deep-red filter used to attenuate flatfield source into broadband imager.

Basler Dart flat field measurement with Aptina 0134 chip was measured in 12-bit and 8-bit mode. Sumix flatfield measurement: due to time constraints, the Sumix demo program was used, which only has 8-bit output to file, despite the Sumix camera having 10-bit output available.

Slide measurement: half the 6 um bead slides at 0.02% concentration had defective preparation, and were discarded. The remaining slides are also somewhat defective, but have enough “good” beads that our cell nucleus detection algorithm picks them out.

Blue fluorescence measurement

Blue excited fluorescence measurement with Polysciences #23519 6 um beads at 0.02% concentration.